DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.
نویسندگان
چکیده
We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at the species and higher taxonomic levels.
منابع مشابه
Redesign of PCR primers for mitochondrial cytochrome c oxidase subunit I for marine invertebrates and application in all-taxa biotic surveys.
DNA barcoding is a powerful tool for species detection, identification and discovery. Metazoan DNA barcoding is primarily based upon a specific region of the cytochrome c oxidase subunit I gene that is PCR amplified by primers HCO2198 and LCO1490 ('Folmer primers') designed by Folmer et al. (Molecular Marine Biology and Biotechnology, 3, 1994, 294). Analysis of sequences published since 1994 ha...
متن کاملMolecular Characterization and Phylogeny Analysis Based on Sequences of Cytochrome Oxidase gene From Hemiscorpius lepturus of Iran
Abstract: Background: Hemiscorpius lepturus is a medically important scorpion found along the Iranian borders, especially near to Khuzestan Province in the south-west of Iran. This is the only non-buthid scorpion which is potentially lethal in southern Iran and is responsible for severe dermonecrotic scorpionism. OBJECTIVES: In this study, DNA fragment of the mitochondrial cytochrome c oxidase ...
متن کاملTaxonomic Position of Iranian Isolates of Eretmocerus mundus (Merect), a Parasitoid of Bemisia tabaci (Gennadius)
Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is one of the most important pest of vegetable and fruit crops. This polyphagous pest has a range of natural enemies including the parasitoid Eretmocerus mundus (Merect) (Hymenoptera: Aphelinidae). To determine the molecular profile and taxonomic status of Iranian isolates of E. mundus, parasitized B. tabaci samples were collected from cotton...
متن کاملPhylogenetic relationships of the commercial marine shrimp family Penaeidae from Persian Gulf
Phylogenetic relationships among all described species (total of 5 taxa) of the shrimp genus Penaeus, were examined with nucleotide sequence data from portions of mitochondrial gene and cytochrome oxidase subunit I (COI). There are twelve commercial shrimp in the Iranian coastal waters. The reconstruction of the evolution phylogeny of these species is crucial in revealing stock identity that ca...
متن کاملStudy of genetic diversity of Dussumieria acuta (Valenciennes, 1847) in Persian Gulf and Oman sea (Coast of the Hormozgan Province) using Cytochrome oxidase subunit I gene (COI)
In this study 12 specimens were collected from Bandar Jask, Qeshm Island and Bandare Lengeh in Hormozgan Province. DNA extraction was performed using Phenol-Chloroform method. A partial DNA sequence of Cytochrome oxidase subunit I gene (COI) was used to evaluate genetic diversity. The sequence of Cytochrome oxidase subunit I gene was done using specific primers designed based on sequences regis...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Molecular marine biology and biotechnology
دوره 3 5 شماره
صفحات -
تاریخ انتشار 1994